Point-of-care blood coagulation monitoring using lateral flow device

Document Type

Conference Proceeding

Publication Title

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014


A simple and affordable method of evaluating blood coagulation ability using microfluidic paperbased lateral flow assay (LFA) device is reported. Rabbit whole blood treated with citrate as anticoagulant was used in experiments. Re-establishment of blood coagulation cascade and creation of different coagulation abilities were achieved by adding CaCl2 solutions of varying concentrations. Since viscosity change of blood depends on its coagulation ability, travel distance of red blood cells (RBC) on nitrocellulose membrane can be used as an indicator of blood clotting time. RBC travel distance is found to decrease linearly with increasing CaCl2 concentration.

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