Selection of Peptide Ligands for Human Placental Transcytosis Systems Using In Vitro Phage Display
Document Type
Article
Publication Title
Methods in Molecular Biology
Abstract
Fetal pharmacotherapy generally relies on nonspecific biodistribution of therapeutic agents to the unborn child following drug administration into the maternal circulation system. Physiologically, transfer of polar, high-molecular weight solutes across the placenta is facilitated by a specialized, vesicular transport mechanism termed transcytosis. To develop biotechnology-based drugs such as proteins, DNA, and siRNA as clinically effective therapeutics, transcytosis systems have been evaluated as a promising strategy to augment drug transfer across endothelial and epithelial barriers. Screening of random peptide libraries using phage display is a powerful technology to identify peptide sequences with high affinity for surface proteins on desired target cells. Here, we describe assembly of a diverse, cyclic heptapeptide library on the icosahedral T7 bacteriophage platform. This phage-displayed library of random peptides was used for functional in vitro screens across BeWo cell monolayers to identify peptide ligands that facilitate placental transcytosis of viral particles across this cell culture model of the human trophoblast barrier.
First Page
141
Last Page
156
DOI
10.1007/978-1-61779-012-6_8
Publication Date
1-1-2011
Recommended Citation
Basha, Saleem; Vaidhyanathan, Shruthi; and Pauletti, Giovanni M., "Selection of Peptide Ligands for Human Placental Transcytosis Systems Using In Vitro Phage Display" (2011). Pharmaceutical and Administrative Sciences Faculty Publications. 168.
https://doi.org/10.1007/978-1-61779-012-6_8
https://collections.uhsp.edu/pharm-admin-sciences_pubs/168