Fluorescent push-pull pH-responsive probes for ratiometric detection of intracellular pH
Document Type
Article
Publication Title
Organic and Biomolecular Chemistry
Abstract
A family of fluorescent push-pull pH-responsive probes based on 2-dicyanomethylidene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran as a strong electron acceptor group is described. Small structural variations allow obtaining pKa ranging from 4.8 to 8.6, underlining the role of the substituent in modulating the acidic properties. Remarkable changes in the optical properties (in particular the fluorescence intensity ratios) were observed as a function of pH. The most interesting probes with pKa close to neutrality were used for ratiometric imaging of intracellular pH. This journal is © the Partner Organisations 2014.
First Page
3641
Last Page
3648
DOI
10.1039/c4ob00147h
Publication Date
6-14-2014
Recommended Citation
Ipuy, Martin; Billon, Cyrielle; Micouin, Guillaume; Samarut, Jacques; Andraud, Chantal; and Bretonnière, Yann, "Fluorescent push-pull pH-responsive probes for ratiometric detection of intracellular pH" (2014). Pharmaceutical and Administrative Sciences Faculty Publications. 67.
https://doi.org/10.1039/c4ob00147h
https://collections.uhsp.edu/pharm-admin-sciences_pubs/67