Mutations in NDUFS1 cause metabolic reprogramming and disruption of the electron transfer
Document Type
Article
Publication Title
Cells
Abstract
Complex I (CI) is the first enzyme of the mitochondrial respiratory chain and couples the electron transfer with proton pumping. Mutations in genes encoding CI subunits can frequently cause inborn metabolic errors. We applied proteome and metabolome profiling of patient-derived cells harboring pathogenic mutations in two distinct CI genes to elucidate underlying pathomechanisms on the molecular level. Our results indicated that the electron transfer within CI was interrupted in both patients by different mechanisms. We showed that the biallelic mutations in NDUFS1 led to a decreased stability of the entire N-module of CI and disrupted the electron transfer between two iron–sulfur clusters. Strikingly interesting and in contrast to the proteome, metabolome profiling illustrated that the pattern of dysregulated metabolites was almost identical in both patients, such as the inhibitory feedback on the TCA cycle and altered glutathione levels, indicative for reactive oxygen species (ROS) stress. Our findings deciphered pathological mechanisms of CI deficiency to better understand inborn metabolic errors.
DOI
10.3390/cells8101149
Publication Date
10-1-2019
Recommended Citation
Ni, Yang; Hagras, Muhammad A.; Konstantopoulou, Vassiliki; Mayr, Johannes A.; Stuchebrukhov, Alexei A.; and Meierhofer, David, "Mutations in NDUFS1 cause metabolic reprogramming and disruption of the electron transfer" (2019). Basic Sciences Faculty Publications. 106.
https://doi.org/10.3390/cells8101149
https://collections.uhsp.edu/basic-sciences_pubs/106