In vitro evolution of enzymes
Document Type
Article
Publication Title
Methods in Molecular Biology
Abstract
In the past decade, in vitro evolution techniques have been used to improve the performance or alter the activity of a number of different enzymes and have generated enzymes de novo. In this review, we provide an overview of the available in vitro methods, their application, and some general considerations for enzyme engineering in vitro. We discuss the advantages of in vitro over in vivo approaches and focus on ribosome display, mRNA display, DNA display technologies, and in vitro compartmentalization (IVC) methods. This review aims to help researchers determine which approach is best suited for their own experimental needs and to highlight that in vitro methods offer a promising route for enzyme engineering. © Springer Science+Business Media New York 2013.
First Page
73
Last Page
92
DOI
10.1007/978-1-62703-293-3_6
Publication Date
1-1-2013
Recommended Citation
Golynskiy, Misha V.; Haugner, John C.; Morelli, Aleardo; Morrone, Dana; and Seelig, Burckhard, "In vitro evolution of enzymes" (2013). Basic Sciences Faculty Publications. 136.
https://doi.org/10.1007/978-1-62703-293-3_6
https://collections.uhsp.edu/basic-sciences_pubs/136