An unexpected diterpene cyclase from rice: Functional identification of a stemodene synthase
Document Type
Article
Publication Title
Archives of Biochemistry and Biophysics
Abstract
We have cloned a novel diterpene synthase (OsKSL11) from rice that produces stemod-13(17)-ene from syn-copalyl diphosphate. Notably, this gene sequence was not predicted from the extensive sequence information available for rice, nor, despite extensive phytochemical investigations, has this diterpene or any derived natural product previously been reported in rice plants. OsKSL11 represents the first identified stemodene synthase, which catalyzes the committed step in biosynthesis of the stemodane family of diterpenoid natural products, some of which possess antiviral activity. In addition, OsKSL11 is highly homologous to the mechanistically similar stemarene synthase recently identified from rice, making this pair of diterpene cyclases an excellent model system for investigating the enzymatic determinants for differential product outcome. The unexpected nature of this cyclase and its product parallels recent observations of previously unrecognized natural products metabolism in Arabidopsis thaliana, suggesting that many, if not all, plant species will prove to have extensive biosynthetic capacity. © 2005 Elsevier Inc. All rights reserved.
First Page
133
Last Page
140
DOI
10.1016/j.abb.2005.09.001
Publication Date
4-15-2006
Recommended Citation
Morrone, Dana; Jin, Yinghua; Xu, Meimei; Choi, Suh Yeon; Coates, Robert M.; and Peters, Reuben J., "An unexpected diterpene cyclase from rice: Functional identification of a stemodene synthase" (2006). Basic Sciences Faculty Publications. 144.
https://doi.org/10.1016/j.abb.2005.09.001
https://collections.uhsp.edu/basic-sciences_pubs/144